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Identification of receptors of the quorum sensing signal autoinducer-2

Catarina S. Pereira^{1, 2}, Anna K. Regt³, Stephen T. Miller³, Karina Bivar Xavier^1,2

¹Instituto Gulbenkian de Ciencia, Portugal; ²Instituto de Tecnologia Quimica Biologica, UNL; ³Swarthmore College, USA;

Altered apoptotic signaling in the yeast model for Batten disease.

Nuno S. Osório¹, Agostinho J. Almeida¹, Agostinho Carvalho², Cecília Leão¹, Nuno Sousa¹, Paula Ludovico¹, João Laranjinha³, Fernando Rodrigues¹

¹Life and Health Sciences Research Institute (ICVS), University of Minho, Portugal; ²Microbiology Section, Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Perugia, Italy; ³Faculty of Pharmacy and Center for Neurosciences and Cell Biology, University of Coimbra, 3000 Coimbra, Portugal;

Phage SPP1 entry into B. subtilis cells: identification of SPP1 tail proteins involved in the recognition of host receptors.

Catarina Baptista¹, Isabelle Auzat², Carlos São-José¹, Mário A. Santos³, Paulo Tavares²

¹Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, Lisbon, Portugal; ²Unité de Virologie Moléculaire et Structurale, CNRS-INRA, Gif-sur-Yvette, France; ³Departamento de Biologia Vegetal, Faculdade de Ciências da Universidade de Lisboa, Lisbon, Portugal;

Transcription regulation in Archaea – characterisation of the virus coded regulator SvtR

Nuno Peixeiro¹, Florence Guillière², Nicole Desnoues¹, J.Iñaki Guijarro², David Prangishvili¹, Guennadi Sezonov¹

¹Institut Pasteur, Unité de Biologie Moléculaire du Gène chez les Extrémophiles, F-75015 Paris, France; ²Institut Pasteur, Unité de RMN des Biomolécules, CNRS URA 2185, F-75015 Paris, France;

Oxidative stress during chronological life span in Saccharomyces cerevisiae

Ana Mesquita¹, Alexandra Silva¹, Belém Sampaio-Marques¹, Bruno Almeida¹, Elsa Logarinho², Cecília Leão¹, Fernando Rodrigues¹, Paula Ludovico¹

¹Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal; ²IBMC, Instituto de Biologia Molecular e Celular, Universidade do Porto, Portugal;

A novel regulator of the expression of beta-lactam resistance in Staphylococcus aureus

Pedro Arede^1,2, Hermínia de Lencastre^2,3, Duarte C. Oliveira^1,2

¹Centro de Recursos Microbiologicos, FCT/UNL,Caparica Portugal; ²Instituto de Tecnologia Quimica e Biologica, FCT/UNL, Oeiras, Portugal; ³Laboratory of Microbiology, The Rockefeller University, New York, NY, USA.;

STRUCTURAL INSTABILITY IN PLASMID VECTORS FOR DNA VACCINATION

Pedro H Oliveira¹, Kristala J Prather², Duarte M Prazeres¹, Gabriel A Monteiro¹

¹IBB, Institute for Biotechnology and Bioengineering, Biological Engineering Research Group, Instituto Superior Técnico, 1049-001 Portugal; ²Department of Chemical Engineering, Massachusetts Institute of Technology, Room 66-458, Cambridge, Massachusetts 02139, USA;

Genome-wide phenotypic and proteomic analyses in Saccharomyces cerevisiae for the identification of novel molecular mechanisms of action and targets of the anti-cancer drug imatinib

Sandra C. dos Santos, Eva R. Sintra, Nuno P. Mira, Isabel Sá-Correia

IBB – Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Técnico, Lisbon, Portugal;

Polysaccharide-based nanoparticles for transmucosal protein delivery

Mafalda Rodrigues, Guilherme N.M. Ferreira, Ana Grenha

Institute for Biotechnology and Bioengineering (IBB), Centre for Molecular and Structural Biomedicine (CBME), University of Algarve, Faro, Portugal;

Evaluation of a novel fusion system for soluble protein overexpression in Escherichia coli

Sofia Judite Marques da Costa^1,2, Pedro Silva^2,3, André Almeida^2,4, António Castro², Lucília Domingues¹

¹Institute for Biotechnology and Bioengineering, Portugal; ²Instituto Nacional de Saúde Dr. Ricardo Jorge - Centro de Saúde Pública Dr. Gonçalves Ferreira, Porto, Portugal; ³Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Portugal; ⁴Hitag Biotechnology, Lda., Portugal;

Colonization of Streptococcus pneumoniae in the era of seven-valent conjugate pneumococcal vaccine

Sónia Nunes¹, Raquel Sá-Leão^1,2, Alexandra S. Simões¹, Nelson Frazão¹, António Brito-Avô³, Hermínia de Lencastre^1,4

¹Laboratory of Molecular Genetics, Instituto de Tecnologia Química e Biológica (ITQB), Universidade Nova de Lisboa, Oeiras, Portugal; ²Centro de Matemática e Aplicações Fundamentais, Universidade de Lisboa, Lisboa, Portugal; ³Centro de Saúde de Oeiras, Oeiras, Portugal; ⁴Laboratory of Microbiology, The Rockefeller University, New York, USA;

Bacterial clone definition using microbial typing methods: Advantages and caveats

João André Carriço

Instituto de Microbiologia, Faculdade de Medicina de Lisboa, Portugal;

Systems Biology for the development of microbial cell factories

Isabel Rocha¹, Anália Lourenço¹, Miguel Rocha², Eugénio C. Ferreira¹

¹IBB - Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal; ²Department of Informatics / CCTC, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal;

Evolutionary analysis of enzymes involved in the synthesis of di myo-inositol-phosphate

Luís G Gonçalves¹, Nuno Borges¹, Pedro Fernandes², Hernán Dopazo³, Helena Santos¹

¹Instituto de Tecnologia Química e Biológica, Portugal; ²Instituto Gulbenkian de Ciência, Portugal; ³Centro de Investigación Príncipe Felipe, Spain;

Global expression analysis of a Staphylococcus aureus mutant with an impaired cell wall

Rita Gonçalves Sobral¹, Ana Madalena Ludovice¹, Alison E. Jones², Shelley G. Des Etages², Thomas J. Dougherty³, Robert M. Peitzsch², Terry Gaasterland⁴, Hermínia De Lencastre^{1, 5}, Alexander Tomasz⁵

¹Laboratory of Molecular Genetics, Instituto de Tecnologia Química e Biológica da Universidade Nova de Lisboa, 2780 Oeiras, Portugal; ²Genetic Technologies, Pfizer Global Research & Development, Groton, CT. 06340; ³Antibacterial Biology, Pfizer Global Research and Development, Groton, CT; ⁴Scripps Institute of Oceanography, La Jolla, Ca. 92093; ⁵The Rockefeller University, 1230 York Avenue, New York, NY 10021;

The regulatory network underlying the transcriptional up-regulation of the FLR1 gene in mancozeb stressed yeast cells: qualitative modeling and simulation

Pedro Tiago Monteiro^2,3, Paulo Jorge Dias¹, Delphine Ropers³, Arlindo Limede Oliveira², Ana Teresa Freitas², Isabel Sá-Correia¹, Miguel Cacho Teixeira¹

¹IBB - Institute for Biotechnology and Bioengineering, CEBQ/IST, Portugal; ²INESC-ID Lisboa / KDBIO, Portugal; ³Institut National de Recherche en Informatique et en Automatique (INRIA), Unité de recherche Rhône-Alpes, France;

Evolutionary Dynamics of Chlamydia trachomatis Key Antigen

Alexandra Nunes, Maria José Borrego, Carlos Florindo, João Paulo Gomes

National Institute of Health, Portugal;

Date: Saturday, 28/11/2009
4:30pm - 6:30pm	Oral Session S1: Microbial Physiology, Molecular Biology and Functional Genomics Session Chair: Helena Santos Session Chair: Margarida Casal
Neptuno
	Identification of receptors of the quorum sensing signal autoinducer-2 Catarina S. Pereira^{1, 2}, Anna K. Regt³, Stephen T. Miller³, Karina Bivar Xavier^1,2 ¹Instituto Gulbenkian de Ciencia, Portugal; ²Instituto de Tecnologia Quimica Biologica, UNL; ³Swarthmore College, USA; Altered apoptotic signaling in the yeast model for Batten disease. Nuno S. Osório¹, Agostinho J. Almeida¹, Agostinho Carvalho², Cecília Leão¹, Nuno Sousa¹, Paula Ludovico¹, João Laranjinha³, Fernando Rodrigues¹ ¹Life and Health Sciences Research Institute (ICVS), University of Minho, Portugal; ²Microbiology Section, Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Perugia, Italy; ³Faculty of Pharmacy and Center for Neurosciences and Cell Biology, University of Coimbra, 3000 Coimbra, Portugal; Phage SPP1 entry into B. subtilis cells: identification of SPP1 tail proteins involved in the recognition of host receptors. Catarina Baptista¹, Isabelle Auzat², Carlos São-José¹, Mário A. Santos³, Paulo Tavares² ¹Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, Lisbon, Portugal; ²Unité de Virologie Moléculaire et Structurale, CNRS-INRA, Gif-sur-Yvette, France; ³Departamento de Biologia Vegetal, Faculdade de Ciências da Universidade de Lisboa, Lisbon, Portugal; Transcription regulation in Archaea – characterisation of the virus coded regulator SvtR Nuno Peixeiro¹, Florence Guillière², Nicole Desnoues¹, J.Iñaki Guijarro², David Prangishvili¹, Guennadi Sezonov¹ ¹Institut Pasteur, Unité de Biologie Moléculaire du Gène chez les Extrémophiles, F-75015 Paris, France; ²Institut Pasteur, Unité de RMN des Biomolécules, CNRS URA 2185, F-75015 Paris, France; Oxidative stress during chronological life span in Saccharomyces cerevisiae Ana Mesquita¹, Alexandra Silva¹, Belém Sampaio-Marques¹, Bruno Almeida¹, Elsa Logarinho², Cecília Leão¹, Fernando Rodrigues¹, Paula Ludovico¹ ¹Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal; ²IBMC, Instituto de Biologia Molecular e Celular, Universidade do Porto, Portugal; A novel regulator of the expression of beta-lactam resistance in Staphylococcus aureus Pedro Arede^1,2, Hermínia de Lencastre^2,3, Duarte C. Oliveira^1,2 ¹Centro de Recursos Microbiologicos, FCT/UNL,Caparica Portugal; ²Instituto de Tecnologia Quimica e Biologica, FCT/UNL, Oeiras, Portugal; ³Laboratory of Microbiology, The Rockefeller University, New York, NY, USA.;

Date: Sunday, 29/11/2009
9:00am - 11:00am	Oral Session S5: Health and Pharmaceutical Biotechnology Session Chair: Virgílio Rosário Session Chair: Arsénio Fialho
Neptuno
	STRUCTURAL INSTABILITY IN PLASMID VECTORS FOR DNA VACCINATION Pedro H Oliveira¹, Kristala J Prather², Duarte M Prazeres¹, Gabriel A Monteiro¹ ¹IBB, Institute for Biotechnology and Bioengineering, Biological Engineering Research Group, Instituto Superior Técnico, 1049-001 Portugal; ²Department of Chemical Engineering, Massachusetts Institute of Technology, Room 66-458, Cambridge, Massachusetts 02139, USA; Genome-wide phenotypic and proteomic analyses in Saccharomyces cerevisiae for the identification of novel molecular mechanisms of action and targets of the anti-cancer drug imatinib Sandra C. dos Santos, Eva R. Sintra, Nuno P. Mira, Isabel Sá-Correia IBB – Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Técnico, Lisbon, Portugal; Polysaccharide-based nanoparticles for transmucosal protein delivery Mafalda Rodrigues, Guilherme N.M. Ferreira, Ana Grenha Institute for Biotechnology and Bioengineering (IBB), Centre for Molecular and Structural Biomedicine (CBME), University of Algarve, Faro, Portugal; Evaluation of a novel fusion system for soluble protein overexpression in Escherichia coli Sofia Judite Marques da Costa^1,2, Pedro Silva^2,3, André Almeida^2,4, António Castro², Lucília Domingues¹ ¹Institute for Biotechnology and Bioengineering, Portugal; ²Instituto Nacional de Saúde Dr. Ricardo Jorge - Centro de Saúde Pública Dr. Gonçalves Ferreira, Porto, Portugal; ³Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Portugal; ⁴Hitag Biotechnology, Lda., Portugal; Colonization of Streptococcus pneumoniae in the era of seven-valent conjugate pneumococcal vaccine Sónia Nunes¹, Raquel Sá-Leão^1,2, Alexandra S. Simões¹, Nelson Frazão¹, António Brito-Avô³, Hermínia de Lencastre^1,4 ¹Laboratory of Molecular Genetics, Instituto de Tecnologia Química e Biológica (ITQB), Universidade Nova de Lisboa, Oeiras, Portugal; ²Centro de Matemática e Aplicações Fundamentais, Universidade de Lisboa, Lisboa, Portugal; ³Centro de Saúde de Oeiras, Oeiras, Portugal; ⁴Laboratory of Microbiology, The Rockefeller University, New York, USA;

Date: Monday, 30/11/2009
9:00am - 11:00am	Oral Session S8: Bioinformatics, Comparative Genomics and Evolution Session Chair: Eugénio Campos Ferreira Session Chair: Mário Ramirez
Neptuno
	Bacterial clone definition using microbial typing methods: Advantages and caveats João André Carriço Instituto de Microbiologia, Faculdade de Medicina de Lisboa, Portugal; Systems Biology for the development of microbial cell factories Isabel Rocha¹, Anália Lourenço¹, Miguel Rocha², Eugénio C. Ferreira¹ ¹IBB - Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal; ²Department of Informatics / CCTC, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal; Evolutionary analysis of enzymes involved in the synthesis of di myo-inositol-phosphate Luís G Gonçalves¹, Nuno Borges¹, Pedro Fernandes², Hernán Dopazo³, Helena Santos¹ ¹Instituto de Tecnologia Química e Biológica, Portugal; ²Instituto Gulbenkian de Ciência, Portugal; ³Centro de Investigación Príncipe Felipe, Spain; Global expression analysis of a Staphylococcus aureus mutant with an impaired cell wall Rita Gonçalves Sobral¹, Ana Madalena Ludovice¹, Alison E. Jones², Shelley G. Des Etages², Thomas J. Dougherty³, Robert M. Peitzsch², Terry Gaasterland⁴, Hermínia De Lencastre^{1, 5}, Alexander Tomasz⁵ ¹Laboratory of Molecular Genetics, Instituto de Tecnologia Química e Biológica da Universidade Nova de Lisboa, 2780 Oeiras, Portugal; ²Genetic Technologies, Pfizer Global Research & Development, Groton, CT. 06340; ³Antibacterial Biology, Pfizer Global Research and Development, Groton, CT; ⁴Scripps Institute of Oceanography, La Jolla, Ca. 92093; ⁵The Rockefeller University, 1230 York Avenue, New York, NY 10021; The regulatory network underlying the transcriptional up-regulation of the FLR1 gene in mancozeb stressed yeast cells: qualitative modeling and simulation Pedro Tiago Monteiro^2,3, Paulo Jorge Dias¹, Delphine Ropers³, Arlindo Limede Oliveira², Ana Teresa Freitas², Isabel Sá-Correia¹, Miguel Cacho Teixeira¹ ¹IBB - Institute for Biotechnology and Bioengineering, CEBQ/IST, Portugal; ²INESC-ID Lisboa / KDBIO, Portugal; ³Institut National de Recherche en Informatique et en Automatique (INRIA), Unité de recherche Rhône-Alpes, France; Evolutionary Dynamics of Chlamydia trachomatis Key Antigen Alexandra Nunes, Maria José Borrego, Carlos Florindo, João Paulo Gomes National Institute of Health, Portugal;