• Department of Chemical Engineering
Project 6 - "Determination of the mass transfer coefficients in a two phase partitioning enzymatic reactor for the degradation of recalcitrant compounds."
| Title of the Project | Determination of the mass transfer coefficients in a two phase partitioning enzymatic reactor for the degradation of recalcitrant compounds. |
| Name of the Institution | Universidade de Santiago de Compostela |
| Town | Santiago de Compostela |
| Country | Spain |
| Host Faculty/Department/ Institute/Laboratory |
Escola Técnica Superior de Enxeñaría Departamento de Enxeñaría Química Grupo de Enxeñaría Ambiental e Bioprocesos |
| Project Supervisor | Juan M. Lema Rodicio |
| Address | rúa Lope Gomez de Marzoa, s/n, 15782 Santiago de Compostela |
| Telephone | +34 981 563.100 ext. 16793 |
| Fax | +34 981 528.050 |
| jmlema@usc.es | |
| Project Objectives | 1- To study the effects of the characteristics of the solvent and the agitation rate on the mass transfer of naphthalene (as an example of recalcitrant compound) between the organic phase (Silicon oil at three different viscosities: 10, 20 and 50 cSt) and the aqueous phase The influence of the presence of surfactants will be also analyzed.. 2- To express the results obtained in point 1 in a non dimensional correlation, in order to make it valid for other type of agitation and reactor. 3- To study the effect of different Reynold’s numbers and surfactant’s concentrations on the enzyme activity and stability, as well as studying different improving techniques. 4- To determine the optimal conditions (organic phase viscosity, w, surfactants) for the degradation process. |
| Required skills | - Good laboratory skills in general. - Know how to work with different computational software: Excel, Table curve 3D… |
| Techniques to be used | - Extraction of anthracene with hexane. - HPLC to follow anthracene’s evolution with time. - GC/MS for determining low concentrations of anthracene (ppb). - Spectrophotometrical determination of enzyme activities. |
| Schedule of Activities | June - Determination of kLa for antrhacene (solvent 1). July- Determination of kLa for anthracene (solvents 2 and 3). August- Effect of mixture, ? and surfactant’s concentration over enzyme activity (solvetn 1). Study of possible stabilizing cofactors for the enzyme. September - Effect of mixture, w and surfactant’s concentration over enzyme activity (solvents 2 and 3). October - Proposal of the model and validation. Optimization of the operation parameters. November- Model justification. |
| Related Bibliography | T.B. Janikowski, D. Velicogna, M. Punt, A.J. Daugulis. 2002. Use of a two-phase partitioning bioreactor for degrading polycyclic aromatic hydrocarbons by a Sphingomonas sp.
Helen Baldascini, Klaassien J. Ganzeveld, Dick B. Janssen, Antonie A. C. M. Beenackers. 2001. Effect of mass transfer limitations on the enzymatic kinetic resolution of epoxides in a two-liquid-phase system. Biotechnology and Bioengineering. 73: 44 – 54 Hans-Jürgen Henzler. 2000. Particle stress in bioreactors. Advances in Biochemical Engineering/Biotechnology. 67:35-80 G. Ascanio, B. Castro, E. Galindo. 2004. Measurement of power consumption in stirred vessels- a review. Trans IChemE. 82 (A9): 1282-1290 |
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